PRODUKSI SAPONIN SEBAGAI ANTIBIOTIK ALAMI dari Aloe Barbadensis Miller : PENANGGULANGAN MASTITIS

Mastitis pada sapi perah terutama disebabkan oleh Staphylococcus aureus. Bakteri tersebut telah multiresisten terhadap antibiotik. Diperlukan antibiotik alami yang diisolasi dari tanaman, yaitu Aloe barbadensis Miller, yang mengandung antibakteri saponin, sebagai pengganti antibiotik sintetis. Penelitian bertujuan menguji aktivitas antibakteri saponin terhadap pertumbuhan Staphylococcus aureus.
Metode penelitian: Digunakan metode eksperimen, estraksi saponin dari gel daun lidah buaya menggunakan larutan n-heksana dan metanol, isolasi saponin dilakukan dengan kromatografi kolom dan kromatografi lapis tipis (KLT). Identifikasi saponin dengan H2SO4, dilanjutkan uji busa dengan pengocokan dalam air, sehingga dihasilkan saponin pekat.
Saponin serbuk diperoleh dengan pengeringan saponin pekat dalam freeze dryer, ditambah bahan pengisi berupa amilum atau dekstrin. Perbandingan antara saponin dengan bahan pengisi adalah 80% : 20%. Enam level saponin yang digunakan adalah 1,5%; 3,0%; 6,2%; 12,5%; 25% dan 50%. Pengujian berupa uji MIC dan cakram. Data hasil penelitian dianalisis secara deskriptif, dengan kontrol positif berupa sediaan penisilin.
Kesimpulan: saponin serbuk memberikan aktivitas antibakteri yang jauh lebih besar daripada saponin pekat. Terdapat kemiripan antara aktivitas antibakteri saponin serbuk yang ditambah bahan pengisi amilum dengan penisilin.
Saponins are a class of chemical compounds, one of many secondary metabolites found in natural sources, with saponins found in particular abundance in various plant species. Specifically, they are amphipathic glycosides grouped phenomenologically by the soap-like foaming they produce when shaken in aqueous solutions, and structurally by their composition of one or more hydrophilic glycoside moieties combined with a lipophilic triterpene derivative.[1][2] A ready and therapeutically relevant example is the cardio-active agent digoxin, from common foxglove
Structural variety and biosynthesis

The aglycone (glycoside-free portion) of the saponins are termed sapogenins. The number of saccharide chains attached to the sapogenin/aglycone core can vary – giving rise to another dimension of nomenclature (monodesmosidic, bidesmosidic, etc.[1]) – as can the length of each chain. A somewhat dated compilation has the range of saccharide chain lengths being 1–11, with the numbers 2-5 being the most frequent, and with both linear and branched chain saccharides being represented.[1] Dietary monosaccharides such as D-glucose and D-galactose are among the most common components of the attached chains.[1]

The lipophilic aglycone can be any one of a wide variety of polycyclic organic structures originating from the serial addition of ten-carbon (C10) terpene units to compose a C30 triterpene skeleton,[3][4] often with subsequent alteration to produce a C27 steroidal skeleton.[1] The subset of saponins that are steroidal have been termed saraponins;[2] Aglycone derivatives can also incorporate nitrogen, so that some saponins also present chemical and pharmacologic characteristics of alkaloid natural products. The figure at right above presents the structure of the alkaloid phytotoxin solanine, a monodesmosidic, branched-saccharide steroidal saponin. (The lipophilic steroidal structure is the series of connected six- and five-membered rings at the right of the structure, while the three oxygen-rich sugar rings are at left and below. Note the nitrogen atom inserted into the steroid skeleton at right.)
[edit] Sources of saponin

Saponins have historically been understood to be plant-derived, but they have also been isolated from marine organisms.[1][5] Saponins are indeed found in many plants,[1][6] and derive their name from the soapwort plant (Genus Saponaria, Family Caryophyllaceae), the root of which was used historically as a soap.[2] Saponins are also found in the botanical family Sapindaceae, with its defining genus Sapindus (soapberry or soapnut), and in the closely related families Aceraceae (maples) and Hippocastanaceae (horse chestnuts; ref. needed). It is also found heavily in gynostemma pentaphyllum (Genus Gynostemma, Family Cucurbitaceae) in a form called gypenosides, and ginseng (Genus Panax, Family Araliaceae) in a form called ginsenosides. Within these families, this class of chemical compounds are found in various parts of the plant: leaves, stems, roots, bulbs, blossom and fruit.[citation needed] Commercial formulations of plant-derived saponins – e.g., from the soap bark (or soapbark) tree, Quillaja saponaria, and from other sources—are available via controlled manufacturing processes, which make them of use as chemical and biomedical reagents.[7]
[edit] Role in plant ecology and impact on animal foraging

In plants, saponins may serve as anti-feedants,[2][4] and to protect the plant against microbes and fungi.[citation needed] Some plant saponins (e.g. from oat and spinach) may enhance nutrient absorption and aid in animal digestion. However, saponins are often bitter to taste, and so can reduce plant palatability (e.g., in livestock feeds), or even imbue them with life-threatening animal toxicity.[4] Data make clear that some saponins are toxic to cold-blooded organisms and insects at particular concentrations.[4] There is a need for further research to define the roles of these natural products in their host organisms—which have been described as "poorly understood" to date.[4]
[edit] Saponins in Ethnobotany

Most saponins, which readily dissolve in water, are poisonous to fish.[8] Therefore, in ethnobotany, saponins are primarily known for their use by indigenous people in obtaining aquatic food sources.

Since prehistoric times, cultures throughout the world have used piscicidal plants, mostly those containing saponins, for fishing.[9][10]

Although prohibited by law, fish poison plants are still widely used by indigenous tribes in Guyana.[11]

On the Indian sub-continent, the Gond tribes are known for their use of plant extracts in poison fishing.[12]
[edit] Established research bioactivities and therapeutic claims
[edit] Bioactivities

One research use of the saponin class of natural products involves their complexation with cholesterol to form pores in cell membrane bilayers, e.g., in red cell (erythrocyte) membranes, where complexation leads to red cell lysis (hemolysis) on intravenous injection.[13] In addition, the amphipathic nature of the class gives them activity as surfactants that can be used to enhance penetration of macromolecules such as proteins through cell membranes.[7] Saponins have also been used as adjuvants in vaccines.[7]

Saponins from the Gypsophila paniculata (Baby’s Breath) plant have been shown to very significantly augment the cytotoxicity of immunotoxins and other targeted toxins directed against human cancer cells. The research groups of Professor Hendrik Fuchs (Charité University, Berlin, Germany) and Dr David Flavell (Southampton General Hospital, United Kingdom) are working together toward the development of Gypsophila saponins for use in combination with immunotoxins or other targeted toxins for patients with leukaemia, lymphoma and other cancers.
[edit] Medical uses

There is tremendous, commercially driven promotion of saponins as dietary supplements and nutriceuticals. There is evidence of the presence of saponins in traditional medicine preparations,[14][15] where oral administrations might be expected to lead to hydrolysis of glycoside from terpenoid (and obviation of any toxicity associated with the intact molecule). But as is often the case with wide-ranging commercial therapeutic claims for natural products:

* the claims for organismal/human benefit are often based on very preliminary biochemical or cell biological studies;[16] and
* mention is generally omitted of the possibilities of individual chemical sensitivity, or to the general toxicity of specific agents,[17]) and high toxicity of selected cases.

While such statements require constant review (and despite the myriad web claims to the contrary), it appears that there are very limited US, EU, etc. agency-approved roles for saponins in human therapy. In their use as adjuvants in the production of vaccines, toxicity associated with sterol complexation remains a major issue for attention.[18] Even in the case of digoxin, therapeutic benefit from the cardiotoxin is a result of careful administration of an appropriate dose. Very great care needs to be exercised in evaluating or acting on specific claims of therapeutic benefit from ingesting saponin-type and other natural products.